5-Methylisoxazole-4-carboxylic acid
5-Methylisoxazole-4-carboxylic acid
CAS:
Molecular Formula: C5H5NO3
5-Methylisoxazole-4-carboxylic acid - Names and Identifiers
5-Methylisoxazole-4-carboxylic acid - Physico-chemical Properties
| Molecular Formula | C5H5NO3 |
| Molar Mass | 127.0981 |
5-Methylisoxazole-4-carboxylic acid - Introduction
5-methylisothiazole-4-carboxylic acid, also known as MTT, is an organic compound. The following is a description of its nature, use, preparation and safety information:
Nature:
-Appearance: White crystalline powder
-Molecular formula: C5H6N2O2S
-Molecular weight: 150.18g/mol
-Melting Point: 97-99°C
-Solubility: MTT has poor solubility in water and is soluble in organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF).
Use:
- MTT can be used as a live cell detection reagent for the evaluation of cell proliferation, cell survival and cytotoxicity.
-It is commonly used in cell activity analysis in cell culture and drug screening to quickly and sensitively detect the metabolic activity of cells.
-In addition, MTT is also used as a catalyst for certain chemical reactions.
Preparation Method:
The preparation of MTT usually has multiple steps:
1. Isothiazole reacts with acetic acid pan-boiling exchanger in chloroform to generate isothiazole -4-formate.
2. The isothiazole -4-formate reacts with acetone to generate 5-methylisothiazole -4-formate.
3. The above product is hydrolyzed with methanol to obtain 5-methylisothiazole-4-carboxylic acid.
Safety Information:
- MTT is generally considered to be relatively safe under normal conditions of use, but it is still necessary to comply with laboratory safety procedures.
-It may be irritating to the skin, eyes and respiratory system, so pay attention to avoid contact during operation. In case of accidental contact, rinse immediately with plenty of water.
-It is recommended to work in a well-ventilated area and to wear suitable protective gloves, goggles and a mask.
Please note that prior to performing the experiment, please understand and follow the relevant laboratory safety rules and regulations in detail.
Nature:
-Appearance: White crystalline powder
-Molecular formula: C5H6N2O2S
-Molecular weight: 150.18g/mol
-Melting Point: 97-99°C
-Solubility: MTT has poor solubility in water and is soluble in organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF).
Use:
- MTT can be used as a live cell detection reagent for the evaluation of cell proliferation, cell survival and cytotoxicity.
-It is commonly used in cell activity analysis in cell culture and drug screening to quickly and sensitively detect the metabolic activity of cells.
-In addition, MTT is also used as a catalyst for certain chemical reactions.
Preparation Method:
The preparation of MTT usually has multiple steps:
1. Isothiazole reacts with acetic acid pan-boiling exchanger in chloroform to generate isothiazole -4-formate.
2. The isothiazole -4-formate reacts with acetone to generate 5-methylisothiazole -4-formate.
3. The above product is hydrolyzed with methanol to obtain 5-methylisothiazole-4-carboxylic acid.
Safety Information:
- MTT is generally considered to be relatively safe under normal conditions of use, but it is still necessary to comply with laboratory safety procedures.
-It may be irritating to the skin, eyes and respiratory system, so pay attention to avoid contact during operation. In case of accidental contact, rinse immediately with plenty of water.
-It is recommended to work in a well-ventilated area and to wear suitable protective gloves, goggles and a mask.
Please note that prior to performing the experiment, please understand and follow the relevant laboratory safety rules and regulations in detail.
Last Update：2024-04-09 20:52:54
